| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1971 | Thermobifida fusca | ACCTTCGCTTCCGCTGAT | TAGCCAGTTCACTGACCGC | 59.02 | 59.71 | 208 | 58 |
100.00%
|
100.00%
|
| Primer 1972 | Thermobifida fusca | ACCTTCGCTTCCGCTGAT | AACAGGGTCATCACTCCGC | 59.02 | 59.70 | 263 | 58 |
100.00%
|
100.00%
|
| Primer 1973 | Thermobifida fusca | ACGGACATGCTCTTCACCG | TGCGCAGTGTAGGTGAGA | 60.08 | 58.23 | 176 | 58 |
100.00%
|
100.00%
|
| Primer 1974 | Thermobifida fusca | AACACTGCCCACGAGCTT | ACCAGGGTCTCGGTGTTGA | 59.49 | 60.46 | 299 | 58 |
100.00%
|
100.00%
|
| Primer 1975 | Thermobifida fusca | AACCAGCCCAACCAGCAA | TCCACCGTGATCAGCCCTT | 59.72 | 60.92 | 269 | 58 |
100.00%
|
100.00%
|
| Primer 1976 | Thermobifida fusca | ACGAGAAAGCCGATGGGA | TCGTGTATTTGGCGACGC | 58.29 | 58.53 | 165 | 58 |
100.00%
|
100.00%
|
| Primer 1977 | Thermobifida fusca | ACGAGAAAGCCGATGGGAC | ATCGTGTATTTGGCGACGC | 59.78 | 59.00 | 166 | 58 |
100.00%
|
100.00%
|
| Primer 1978 | Thermobifida fusca | TAGCTGTGATGACCGTCGC | TTTGACGAGTGCGCTGCT | 59.86 | 60.28 | 278 | 58 |
100.00%
|
100.00%
|
| Primer 1979 | Thermobifida fusca | GCGATCCTCAACGAGCAGA | AGGCTCTGGTAGATGGGCA | 59.86 | 60.00 | 182 | 58 |
100.00%
|
100.00%
|
| Primer 1980 | Thermobifida fusca | TCAGTGTCCTGTTCACCGC | TAGACGTGGTGCAGTTCCG | 59.93 | 59.71 | 193 | 58 |
100.00%
|
100.00%
|